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D Pharm 2nd Year

As Per New Syllabus [ ER 20]

Biochemistry & Clinical Pathology

Notes



Chapter 1​

Introduction to biochemistry: Scope of biochemistry in pharmacy; Cell and its biochemical organization.

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Chapter 2​

Carbohydrates

Definition, classification with examples, chemical properties

Monosaccharides – Structure of glucose, fructose, and galactose

Disaccharides – structure of maltose, lactose, and sucrose

Polysaccharides – chemical nature of starch and glycogen

Qualitative tests and biological role of carbohydrates

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Chapter 3​

Proteins

Definition, classification of proteins based on composition and solubility with examples

Definition, classification of amino acids based on chemical nature and nutritional requirements with examples

Structure of proteins (four levels of organization of protein structure)

Qualitative tests and biological role of proteins and amino acids

Diseases related to malnutrition of proteins.

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Chapter 4​

Lipids

Definition, classification with examples

Structure and properties of triglycerides (oils and fats)

Fatty acid classification – Based on chemical and nutritional requirements with examples

Structure and functions of cholesterol in the body

Lipoproteins – types, composition and functions in the body

Qualitative tests and functions of lipids

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Chapter 5​

Nucleic acids

Definition, purine and pyrimidine bases

Components of nucleosides and nucleotides with examples

Structure of DNA (Watson and Crick model), RNA and their functions

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Chapter 6​

Enzymes

Definition, properties and IUB and MB classification

Factors affecting enzyme activity

Mechanism of action of enzymes, Enzyme inhibitors

Therapeutic and pharmaceutical importance of enzymes

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Chapter 7​

Vitamins

Definition and classification with examples

Sources, chemical nature, functions, coenzyme form, recommended dietary requirements, deficiency

diseases of fat-and water-soluble vitamins

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Chapter 8​

Metabolism (Study of cycle/pathways without chemical structures)

Metabolism of Carbohydrates: Glycolysis, TCA cycle

and glycogen metabolism, regulation of blood glucose

level. Diseases related to abnormal metabolism of

Carbohydrates

Metabolism of lipids: Lipolysis, β-oxidation of Fatty acid

(Palmitic acid) ketogenesis and ketolysis. Diseases

related to abnormal metabolism of lipids such as

Ketoacidosis, Fatty liver, Hypercholesterolemia

Metabolism of Amino acids (Proteins): General

reactions of amino acids and its significance–

Transamination, deamination, Urea cycle and

decarboxylation. Diseases related to abnormal

metabolism of amino acids, Disorders of ammonia

metabolism, phenylketonuria, alkaptonuria and

Jaundice.

Biological oxidation: Electron transport chain

and Oxidative phosphorylation

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Chapter 9​

Minerals: Types, Functions, Deficiency diseases,

recommended dietary requirements

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Chapter 10​

Water and Electrolytes

Distribution, functions of water in the body

Water turnover and balance

Electrolyte composition of the body fluids, Dietary intake of electrolyte and Electrolyte balance

Dehydration, causes of dehydration and oral rehydration therapy

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Chapter 11​

Introduction to Biotechnology

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Chapter 12​

Organ function tests

Functions of kidney and routinely performed tests to

assess the functions of kidney and their clinical

significances

Functions of liver and routinely performed tests to

assess the functions of liver and their clinical

significances

Lipid profile tests and its clinical significances

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Chapter 13​

Introduction to Pathology of Blood and Urine

Lymphocytes and Platelets, their role in health and disease

Erythrocytes – Abnormal cells and their significance

Normal and Abnormal constituents of Urine and theirsignificance

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