Unit 1

Nuclear Magnetic Resonance spectroscopy  

Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications Mass Spectrometry

Notes-1

Mass Spectrometry

Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

Notes-2

Unit 2

Thermal Methods of Analysis

Principles, instrumentation and applications of Thermogravimetric Analysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC) 

Notes-1

X-Ray Diffraction Methods

Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.

Notes-2

Unit 3

Calibration and validation

as per ICH and USFDA guidelines 

Calibration of following Instruments 

Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC

Unit 4

Radio immune assay

Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay 

Notes-1

Extraction techniques

General principle and procedure involved in the solid phase extraction and liquid-liquid extraction

Unit 5

Hyphenated techniques

LC-MS/MS, GC-MS/MS, HPTLC-MS.

Notes